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Amersham Life Sciences Inc nylon membrane 273
Nylon Membrane 273, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nylon+membrane/membrane+nylon/pm42302291-127-8-12
Average 86 stars, based on 1 article reviews
nylon membrane 273 - by Bioz Stars, 2026-09
86/100 stars

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Membrane:

Article Title: Ribosomal RNA processing undergoes surveillance by the mRNA guard protein Npl3.
Article Snippet: .. After another rinse in DEPC treated water, the gel was soaked for 20 min in 6 or 10 × SSC (1.5 M NaCl, 150 mM sodium citrate, pH 7.0) and RNA was transferred to positively charged nylon membrane (Amersham Hybond -N+ (GE Healthcare) over night by capillary blotting or in 2 h using a vacuum blotting system. ..

Article Title: An HBV‐Derived Peptide Poly6 as a Novel Candidate for Functional Cure Via IFN‐I–Mediated Epigenetic Regulation of cccDNA
Article Snippet: .. PCR products were resolved on a 1.2% agarose gel at 50 V and transferred to a nylon membrane (Hybond N + , Amersham, UK). ..

Article Title: KIAA1199 aggravates sepsis-induced lung injury by promoting complement activation.
Article Snippet: .. After incubation for 20 min at room temperature, the reaction mixtures were then separated by electrophoresis with 6% non-denaturing polyacrylamide gel in 0.5×trisborate-EDTA buffer (pH 8.3) at 100 V for 1 h. The bound and free probes in the gel were transferred to a nylon membrane (Amersham, USA) at 380 mA for 1 h. The probes on the membrane were detected using chemiluminescent EMSA kit (GS009, Beyotime) as instructed. ..

Article Title: Conditional deletion of human STN1 leads to telomere dysfunction, genome instability and proliferation defects.
Article Snippet: .. The digested DNA was then run on an 0.8% 1× TrisAcetate-EDTA (TAE) gel for 6 h at 50 V. The gel was depurinated, denatured and neutralized, as previously described, and the DNA transferred to a nylon membrane (Hybond N+, Amersham) by capillary action overnight. .. The membrane was pre-hybridized for at least 3 h at 60°C followed by hybridization with a 3′-digoxigenin (DIG)-labeled (CCCTAA)3 probe (Integrated DNA Technologies) at 60°C overnight.

Article Title: A class II 5-enolpyruvylshikimate-3-phosphate synthase from Pseudomonas P818 confers robust glyphosate tolerance in transgenic plants
Article Snippet: .. The digested DNA was separated by electrophoresis on 0.8 % agarose gels and transferred to a nylon membrane (Amersham). .. The membrane was hybridized with DIG-labeled probes using a DIG High Primer DNA Labeling and Detection Starter Kit III (Roche).

Article Title: Non-enzymatic RNA Glycation is a Metabolic Sensor of Cellular Stress
Article Snippet: .. Ribosome footprints were purified using TRIzol according to the manufacturer’s instruction and ran on 15% TBE-urea gel followed by transferring to a nylon membrane (Amersham Hybond-N+, RPN203B) for autoradiography. .. Radioactive signals were visualized using an Amersham Typhoon 5.

Article Title: AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet
Article Snippet: .. For northern blots, 15 μg of RNA was used for electrophoresis on an 8 M 10% TBE urea gel, and the gels were subsequently transferred onto a positively charged nylon membrane (GE Healthcare, Amersham, UK; #RPN303B), followed by UV-crosslinking with an energy of 120 mJ. .. The membranes were pre-hybridized with Ultrahyb-oligo buffer (Thermo Fisher Scientific, Waltham, MA, USA; #8663) for 30 min, rotating in a hybridization oven at 42 °C.

Article Title: Compositions and methods for long term culture of hepatocytes
Article Snippet: .. For Southern Blot, the prepared DNA sample was separated on a 0.8% agarose gel and then transferred to a nylon membrane (Amersham). .. Real-Time PCRs were carried out using Power SYBR Green PCR Master Mix (Applied Biosystems) and performed on a CFX384 Real-Time PCR detection system (Bio-Rad).

Polymerase Chain Reaction:

Article Title: An HBV‐Derived Peptide Poly6 as a Novel Candidate for Functional Cure Via IFN‐I–Mediated Epigenetic Regulation of cccDNA
Article Snippet: .. PCR products were resolved on a 1.2% agarose gel at 50 V and transferred to a nylon membrane (Hybond N + , Amersham, UK). ..

Agarose Gel Electrophoresis:

Article Title: An HBV‐Derived Peptide Poly6 as a Novel Candidate for Functional Cure Via IFN‐I–Mediated Epigenetic Regulation of cccDNA
Article Snippet: .. PCR products were resolved on a 1.2% agarose gel at 50 V and transferred to a nylon membrane (Hybond N + , Amersham, UK). ..

Article Title: Compositions and methods for long term culture of hepatocytes
Article Snippet: .. For Southern Blot, the prepared DNA sample was separated on a 0.8% agarose gel and then transferred to a nylon membrane (Amersham). .. Real-Time PCRs were carried out using Power SYBR Green PCR Master Mix (Applied Biosystems) and performed on a CFX384 Real-Time PCR detection system (Bio-Rad).

Incubation:

Article Title: KIAA1199 aggravates sepsis-induced lung injury by promoting complement activation.
Article Snippet: .. After incubation for 20 min at room temperature, the reaction mixtures were then separated by electrophoresis with 6% non-denaturing polyacrylamide gel in 0.5×trisborate-EDTA buffer (pH 8.3) at 100 V for 1 h. The bound and free probes in the gel were transferred to a nylon membrane (Amersham, USA) at 380 mA for 1 h. The probes on the membrane were detected using chemiluminescent EMSA kit (GS009, Beyotime) as instructed. ..

Electrophoresis:

Article Title: KIAA1199 aggravates sepsis-induced lung injury by promoting complement activation.
Article Snippet: .. After incubation for 20 min at room temperature, the reaction mixtures were then separated by electrophoresis with 6% non-denaturing polyacrylamide gel in 0.5×trisborate-EDTA buffer (pH 8.3) at 100 V for 1 h. The bound and free probes in the gel were transferred to a nylon membrane (Amersham, USA) at 380 mA for 1 h. The probes on the membrane were detected using chemiluminescent EMSA kit (GS009, Beyotime) as instructed. ..

Article Title: A class II 5-enolpyruvylshikimate-3-phosphate synthase from Pseudomonas P818 confers robust glyphosate tolerance in transgenic plants
Article Snippet: .. The digested DNA was separated by electrophoresis on 0.8 % agarose gels and transferred to a nylon membrane (Amersham). .. The membrane was hybridized with DIG-labeled probes using a DIG High Primer DNA Labeling and Detection Starter Kit III (Roche).

Article Title: AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet
Article Snippet: .. For northern blots, 15 μg of RNA was used for electrophoresis on an 8 M 10% TBE urea gel, and the gels were subsequently transferred onto a positively charged nylon membrane (GE Healthcare, Amersham, UK; #RPN303B), followed by UV-crosslinking with an energy of 120 mJ. .. The membranes were pre-hybridized with Ultrahyb-oligo buffer (Thermo Fisher Scientific, Waltham, MA, USA; #8663) for 30 min, rotating in a hybridization oven at 42 °C.

Purification:

Article Title: Non-enzymatic RNA Glycation is a Metabolic Sensor of Cellular Stress
Article Snippet: .. Ribosome footprints were purified using TRIzol according to the manufacturer’s instruction and ran on 15% TBE-urea gel followed by transferring to a nylon membrane (Amersham Hybond-N+, RPN203B) for autoradiography. .. Radioactive signals were visualized using an Amersham Typhoon 5.

Transferring:

Article Title: Non-enzymatic RNA Glycation is a Metabolic Sensor of Cellular Stress
Article Snippet: .. Ribosome footprints were purified using TRIzol according to the manufacturer’s instruction and ran on 15% TBE-urea gel followed by transferring to a nylon membrane (Amersham Hybond-N+, RPN203B) for autoradiography. .. Radioactive signals were visualized using an Amersham Typhoon 5.

Autoradiography:

Article Title: Non-enzymatic RNA Glycation is a Metabolic Sensor of Cellular Stress
Article Snippet: .. Ribosome footprints were purified using TRIzol according to the manufacturer’s instruction and ran on 15% TBE-urea gel followed by transferring to a nylon membrane (Amersham Hybond-N+, RPN203B) for autoradiography. .. Radioactive signals were visualized using an Amersham Typhoon 5.

Northern Blot:

Article Title: AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet
Article Snippet: .. For northern blots, 15 μg of RNA was used for electrophoresis on an 8 M 10% TBE urea gel, and the gels were subsequently transferred onto a positively charged nylon membrane (GE Healthcare, Amersham, UK; #RPN303B), followed by UV-crosslinking with an energy of 120 mJ. .. The membranes were pre-hybridized with Ultrahyb-oligo buffer (Thermo Fisher Scientific, Waltham, MA, USA; #8663) for 30 min, rotating in a hybridization oven at 42 °C.

Southern Blot:

Article Title: Compositions and methods for long term culture of hepatocytes
Article Snippet: .. For Southern Blot, the prepared DNA sample was separated on a 0.8% agarose gel and then transferred to a nylon membrane (Amersham). .. Real-Time PCRs were carried out using Power SYBR Green PCR Master Mix (Applied Biosystems) and performed on a CFX384 Real-Time PCR detection system (Bio-Rad).



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